Determining Amino Acid Composition
It is often desirable to know the unordered amino acid composition of a protein prior to attempting to find the ordered sequence, as this knowledge can be used to facilitate the discovery of errors in the sequencing process or to distinguish between ambiguous results. Knowledge of the frequency of certain amino acids may also be used to choose which protease to use for digestion of the protein. A generalized method often referred to as amino acid analysis for determining amino acid frequency is as follows:
- Hydrolyse a known quantity of protein into its constituent amino acids.
- Separate the amino acids in some way.
Read more about this topic: Protein Sequencing
Famous quotes containing the words determining and/or composition:
“Our civilization has decided ... that determining the guilt or innocence of men is a thing too important to be trusted to trained men.... When it wants a library catalogued, or the solar system discovered, or any trifle of that kind, it uses up its specialists. But when it wishes anything done which is really serious, it collects twelve of the ordinary men standing round. The same thing was done, if I remember right, by the Founder of Christianity.”
—Gilbert Keith Chesterton (1874–1936)
“It is my PRIDE, my damn’d, native, unconquerable Pride, that plunges me into Distraction. You must know that 19-20th of my Composition is Pride. I must either live a Slave, a Servant; to have no Will of my own, no Sentiments of my own which I may freely declare as such;Mor DIE—perplexing alternative!”
—Thomas Chatterton (1752–1770)