Protein Extraction From Tissues
Protein extraction from tissues with tough extracellular matrices (biopsy samples, venous tissues, cartilage, skin) is often achieved in a laboratory setting by impact pulverization in liquid nitrogen. Samples are frozen in liquid nitrogen and subsequently subjected to impact or mechanical grinding. As water in the samples becomes very brittle at these temperature, the samples are often reduced to a collection of fine fragments, which can then be dissolved for protein extraction. Stainless steel devices known as tissue pulverizers are sometimes used for this purpose (Protein Extraction Tissue Pulverizer). Advantages of these devices include high levels of protein extraction from small, valuable samples, disadvantages include low-level cross-over contamination.
Read more about this topic: Protein Methods
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