Primer extension is a technique whereby the 5' ends of RNA or DNA can be mapped.
Primer extension can be used to determine the start site of RNA transcription for a known gene. This technique requires a radiolabelled primer (usually 20 - 50 nucleotides in length) which is complementary to a region near the 3' end of the gene. The primer is allowed to anneal to the RNA and reverse transcriptase is used to synthesize cDNA from the RNA until it reaches the 5' end of the RNA. By running the product on a polyacrylamide gel, it is possible to determine the transcriptional start site, as the length of the sequence on the gel represents the distance from the start site to the radiolabelled primer.
Famous quotes containing the words primer and/or extension:
“Your master Robin Hood lies dead,
Therefore sigh as you sing.
Here lie his primer and his beads,
His bent bow and his arrows keen,
His good sword and his holy cross:”
—Anthony Munday (1553–1633)
“The motive of science was the extension of man, on all sides, into Nature, till his hands should touch the stars, his eyes see through the earth, his ears understand the language of beast and bird, and the sense of the wind; and, through his sympathy, heaven and earth should talk with him. But that is not our science.”
—Ralph Waldo Emerson (1803–1882)