The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the bacterial periplasm, where the sequence is removed by a signal peptidase. Specifically, pelB refers to pectate lyase B of Erwinia carotovora CE. The leader sequence consists of the 22 N-terminal amino acid residues. This leader sequence can be attached to any other protein (on the DNA level) resulting in a transfer of such a fused protein to the periplasmic space of Gram-negative bacteria, such as Escherichia coli, often used in genetic engineering. protein secretion can increase the stability of cloned gene products.For instance it was shown that the half-life of the recombinant proinsulin is increased 10-fold when the protein is secreted to the periplasmic space. (vijji.Narne, R.S.Ramya)
One of possible applications is to direct coat protein-antigen fusions to the cell surface for construction of engineered bacteriophage for the purpose of phage display.
- SP Lei et al., Characterization of the Erwinia carotovora pelB gene and its product pectate lyase. J. Bacteriol. 169(9): 4379–4383 (1987).
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