Example
In part 1a of the schematic the oligonucleotide probe, labeled on its left end (asterisk), is shown on the top line. It is fully complementary to its target DNA (here taken from the human β-hemoglobin gene), as shown on the next line. Part of the probe includes the Recognition site for the restriction enzyme Dde I (underlined).
In part 1b, the restriction enzyme has cleaved the probe and its target (Dde I leaves three bases unpaired at each end). The labeled end of the probe is now just 8 bases in length, and is easily separated by Gel electrophoresis from the uncut probe, which was 40 bases long.
In part 2, the same probe is shown hybridized to a target DNA which includes a single base mutation (here the mutation responsible for Sickle Cell Anemia, or SCA). The mismatched hybrid no longer acts as a recognition site for the restriction enzyme, and the probe remains at its original length.
Read more about this topic: Oligomer Restriction
Famous quotes containing the word example:
“Our intellect is not the most subtle, the most powerful, the most appropriate, instrument for revealing the truth. It is life that, little by little, example by example, permits us to see that what is most important to our heart, or to our mind, is learned not by reasoning but through other agencies. Then it is that the intellect, observing their superiority, abdicates its control to them upon reasoned grounds and agrees to become their collaborator and lackey.”
—Marcel Proust (1871–1922)