Nucleic Acid Methods

Nucleic acid methods are the techniques used to study nucleic acids (DNA and RNA).

Purification
  • Phenol-chloroform extraction
  • minicolumn purification
  • RNA extraction
  • Boom method
Quantification
  • Abundance in weight: spectroscopic quantification
  • Absolute abundance in number: Q-PCR
  • high-throughput relative abundance DNA microarray
  • high-throughput absolute abundance SAGE
  • Size: Gel electrophoresis
Synthesis
  • De novo: Oligonucleotide synthesis
  • Amplification: PCR
Kinetics
  • Dual polarization interferometry
  • QCM-D
Other
  • Nucleic acid simulations
  • DNA sequencing
  • Bisulfite sequencing
  • Expression cloning
  • Southern blot
  • Northern blot
  • Sucrose gradient centrifugation
  • Radioactivity in biological research
  • Lab-on-a-chip
  • Nuclear run-on assay
  • Fluorescent in situ hybridization
  • several Bioinformatics methods, such as RNA structure prediction

Read more about Nucleic Acid Methods:  See Also

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    François, Duc De La Rochefoucauld (1613–1680)