Mir-10 Micro RNA Precursor Family - Targets of MiR-10

Targets of MiR-10

A number of Hox genes have been shown to be regulated by miR-10. These genes encode transcription factors which are important in embryonic development. In zebrafish embryos, miR-10 binds to sites in the three prime untranslated region (3'UTR) of the HoxB1a and HoxB3a genes, which are important in anterior-posterior patterning during embryonic development. Binding of miR-10 leads to the repression of these genes. It also acts synergistically with HoxB4 to repress these genes. The mir-10 gene is located near to the HoxB1a and HoxB3a genes within the zebrafish genome, Hox-1 and Hox-3 paralogues located on different Hox clusters are not targets of miR-10. Human HOXD10 gene has also been shown experimentally to be repressed by miR-10a and miR-10b.

It has also been experimentally verified that miR-10a downregulates the human HOXA1 and HOXA3 genes. Control of the Hox genes by miR-10 suggests that this microRNA may play an important role in development.

In addition to the Hox genes, miR-10a represses the transcription factor USF2 and the Ran and Pbp1 genes. The cell-surface proteoglycan Syndecan-1 is a target of miR-10b.

miR-10a binds to the five prime untranslated region (5'UTR) of mRNAs encoding ribosomal proteins, and increases their translation. It binds immediately downstream of the 5' oligopyrimidine tract (5'TOP) motif, a region important in the regulation of ribosomal protein synthesis.