John Gardiner may refer to:
- John Gardiner, Baron Gardiner of Kimble (born 1956), British peer
- John Sylvester John Gardiner (1765–1830), Rector of Trinity Church, Boston, Massachusetts
- John Gardiner (Australia) (1798–1878), banker and grazier
- John Gardiner (footballer) (born 1958), Scottish footballer
- John Gardiner (footballer born 1911), Scottish footballer
- John Gardiner (hurler) (born 1983), hurler with Cork GAA
- Sir John Eliot Gardiner (born 1943), British conductor
- John Reynolds Gardiner (1944–2006), American children's author
- John Gardiner (Montreal politician), former politician in Montreal, Quebec, Canada
- John Stanley Gardiner (1872–1946), British zoologist
- John Gardiner (died 1586), MP for Penryn and Dorchester