General Scheme
The HSQC experiment is a highly-sensitive 2D-NMR experiment and was first described in a 1H–15N system, but is also applicable to other nuclei such as 1H–13C system. The basic scheme of this experiment involves the transfer of magnetization on the proton to the second nucleus, which may be 15N or 13C, via an INEPT (Insensitive nuclei enhanced by polarization transfer) step. After a time delay (t1), the magnetization is transferred back to the proton via retro-INEPT step and the signal is then recorded. In HSQC, a series of experiment is recorded where the time delay t1 is incremented. The 1H signal is detected in the directly measured dimension in each experiment, while chemical shift of 15N or 13C is recorded in the indirect dimension which is formed from the series of experiments.
Read more about this topic: Heteronuclear Single Quantum Coherence Spectroscopy
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