GUS
- Gravis Ultrasound, a sound card for PCs
- GUS (retailer), a British retailing company
- Central Statistical Office, Poland (Główny Urząd Statystyczny), the Polish national statistics office
- Gul Ursani School, Qasimabad, Hyderabad, Sindh.
- Gulder Ultimate Search, a Nigerian reality show
- beta-glucuronidase, a lysosomal enzyme
- GUS reporter system (also: GUS Staining, GUS Assay) a molecular biology technique
- Gemeinschaft Unabhängiger Staaten, the Commonwealth of Independent States, a modern-day political entity consisting of 11 former Soviet Union Republics
- Groupes urbains de sécurité, a Moroccan police unit
- GUS, designation of slow westbound UG convoys in World War II
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