History
Paraffin tissue array was developed during late years in the 1980s; this array can help scientists high throughput analyze gene and protein expressions in multiple tissue samples, especially analyze different protein levels with antibodies by immunohistochemistry. Various paraffin tissue arrays are now commercially available from many biotech companies. Most of the arrays can be easily made by microarraying instrument (Beecher Instruments Inc.). However, paraffin embedded tissues have limitations. Buffered formalin solutions cross link proteins and nucleic acids when they are used for fix tissues. The DNA, RNA, and protein within the tissues are damaged in various levels during the fixation. Therefore, lots of scientific experimental results from formalin fixed tissues are not reliable. Since frozen tissue sections don’t go through any fixation procedures, and therefore, the DNA, RNA, and protein in frozen tissues retain their native characteristics much more than in paraffin embedded tissues. Scientists who have been developing antibodies for therapeutic purpose all need their preliminary results from frozen tissues to get approval from FDA. Consequently frozen tissue array should be the best tool for high throughput analysis on this purpose.
Read more about this topic: Frozen Tissue Array
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