Software
| Name | Primary author | Platform | License |
|---|---|---|---|
| Adobe Photoshop CS5 | Adobe | Windows, Mac OS X | Proprietary |
| ImageFocus Stacking software | Euromex Microscopes Holland | Windows | Proprietary, 30-day trial |
| ALE | David Hilvert | Linux, Windows | GPL |
| CombineZ | Alan Hadley | Windows | GPL |
| Deep Focus module for QuickPHOTO | PROMICRA | Windows | Proprietary, time-unlimited trial |
| Enfuse (combined with align_image_stack or similar) | Andrew Mihal and hugin development team | Multiplatform | GPL |
| Extended Depth of Field plugin for ImageJ |
Alex Prudencio, Jesse Berent and Daniel Sage | Multi-platform (Java) | Free for use for research purposes |
| Helicon Focus | Danylo Kozub | Windows, Mac OS X | Proprietary, 30-day trial |
| Image Pro Plus | Media Cybernetics | Windows | Proprietary |
| Macnification | Peter Schols | Mac OS X | Proprietary, 30-day trial |
| MacroFusion, GUI for Enfuse | Dariusz Duma | Linux | GPL v2 |
| PhotoAcute Studio | Almalence Inc | Windows, Mac OS X, Linux | Proprietary, time-unlimited trial |
| PICOLAY | Heribert Cypionka | Windows | Proprietary |
| Stack Focuser plugin for ImageJ |
Michael Umorin | Multi-platform (Java) | GPL |
| Tufuse | Max Lyons | Windows | Freeware |
| Zeiss Axiovision | Carl Zeiss AG | Windows | |
| Zerene Stacker | Rik Littlefield | Windows, Mac OS X, Linux | Proprietary, 30-day trial |
Read more about this topic: Focus Stacking