Summary of Properties and Advantages of ENU Mutagenesis
- ENU is an alkylating agent and has preference for A->T base transversions and also for AT->GC transitions. However it is also shown to cause GC->AT transitions.
- It is known to induce point mutations, which implies that by mapping for the desired phenotype, the researcher can identify a single candidate gene responsible for the phenotype.
- The point mutations are at approximately 1-2 Mb interval and occur at an approximate rate of 1 per 700 gametes.
- Point mutations induced by ENU can either be gain-of-function mutations or loss-of function mutations in a gene as against deletions, which can induce only loss-of-function mutations.
- ENU targets spermatogonial stem cells.
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