Diatoms - EST Sequencing

EST Sequencing

The first insights into the properties of the P. tricornutum gene repertoire were described using 1,000 ESTs. Subsequently, the number of ESTs was extended to 12,000 and the Diatom EST Database was constructed for functional analyses. These sequences have been used to make a comparative analysis between P. tricornutum and the putative complete proteomes from the green alga Chlamydomonas reinhardtii, the red alga Cyanidioschyzon merolae, and T. pseudonana. The diatom EST database now consists of over 200,000 ESTs from P. tricornutum (16 libraries) and T. pseudonana (7 libraries) cells grown in a range of different conditions, many of which correspond to different abiotic stresses (available at http://www.biologie.ens.fr/diatomics/EST3/).