Measurement of Chemotaxis
A wide range of techniques is available to evaluate chemotactic activity of cells or the chemoattractant and chemorepellent character of ligands. The basic requirements of the measurement are as follows:
- concentration gradients can develop relatively fast and persist for a long time in the system
- chemotactic and chemokinetic activities are distinguished
- migration of cells is free towards and away on the axis of the concentration gradient
- detected responses are the results of active migration of cells
Despite the fact that an ideal chemotaxis assay is still not available, there are several protocols and pieces of equipment which offer good correspondence with the conditions described above. The most commonly used are summarised in the table below:
| Type of assay | Agar-plate assays | Two-chamber assays | Others |
|---|---|---|---|
| Examples |
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Read more about this topic: Chemotaxis
Famous quotes containing the words measurement of and/or measurement:
“That’s the great danger of sectarian opinions, they always accept the formulas of past events as useful for the measurement of future events and they never are, if you have high standards of accuracy.”
—John Dos Passos (1896–1970)
“That’s the great danger of sectarian opinions, they always accept the formulas of past events as useful for the measurement of future events and they never are, if you have high standards of accuracy.”
—John Dos Passos (1896–1970)