Charles Johnston may refer to:
- Charles Johnston, Baron Johnston of Rockport (1915–2002), British politician
- Charles Clement Johnston (1795–1832), U.S. Representative from Virginia
- Charles E. Johnston (1881–1951), former president of Kansas City Southern Railway
- Charles H. Johnston, U.S. admiral, retired 2005
- Charles John Johnston (1845–1918), Mayor of Wellington, New Zealand
- Charles Edward Johnston (1899–1971), long serving Canadian federal politician
- Charles Hepburn Johnston (1912–1986), British diplomat and author who translated Pushkin's Eugene Onegin
- Charles Johnston (representative) (1793–1845), U.S. Representative from New York
- Charles Johnston (umpire) (1896–1988), Major League Baseball umpire
- Sir Charles Johnston, 1st Baronet (1848–1933), Lord Mayor of London between 1914 and 1915
- Charles Johnston (captive of Native Americans) (c. 1770–1833), American lawyer who wrote a captivity narrative