Performance
Bright field microscopy typically has low contrast with most biological samples as few absorb light to a great extent. Staining is often required to increase contrast, which prevents use on live cells in many situations. Bright field illumination is useful for samples which have an intrinsic colour, for example chloroplasts in plant cells.
- Comparison of transillumination techniques used to generate contrast in a sample of tissue paper. 1.559 μm/pixel.
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Bright field illumination, sample contrast comes from absorbance of light in the sample.
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Cross-polarized light illumination, sample contrast comes from rotation of polarized light through the sample.
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Dark field illumination, sample contrast comes from light scattered by the sample.
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Phase contrast illumination, sample contrast comes from interference of different path lengths of light through the sample.
Bright field microscopy is a standard light microscopy technique and therefore magnification is limited by the resolving power possible with the wavelength of visible light.
Read more about this topic: Bright Field Microscopy
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