Blue White Screen
The blue-white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. DNA of interest is ligated into a vector. The vector is then transformed into competent cell (bacteria), and the competent cells are grown in the presence of X-gal. Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.
Read more about Blue White Screen: Background, Molecular Mechanism, Practical Considerations
Famous quotes containing the words blue and/or white:
“One way to do it might be by making the scenery penetrate the automobile. A polished black sedan was a good subject, especially if parked at the intersection of a tree-bordered street and one of those heavyish spring skies whose bloated gray clouds and amoeba-shaped blotches of blue seem more physical than the reticent elms and effusive pavement. Now break the body of the car into separate curves and panels; then put it together in terms of reflections.”
—Vladimir Nabokov (1899–1977)
“Slow, slow, as a fish she came,
Slow as a fish coming forward,
Swaying in a long wave;
Her skirts not touching a leaf,
Her white arms reaching towards me.”
—Theodore Roethke (1908–1963)