Biological Small-angle Scattering - Biological Molecule Layers and GISAS

Biological Molecule Layers and GISAS

Coatings of biomolecules can be studied with grazing-incidence X-ray and neutron scattering. IsGISAXS (Grazing Incidence Small Angle X-ray Scattering) is a software program dedicated to the simulation and analysis of GISAXS from nanostructures. IsGISAXS only encompasses the scattering by nanometric sized particles, which are buried in a matrix subsurface or supported on a substrate or buried in a thin layer on a substrate. The case of holes is also handled. The geometry is restricted to a plane of particles. The scattering cross section is decomposed in terms of interference function and particle form factor. The emphasis is put on the grazing incidence geometry which induces a "beam refraction effect". The particle form factor is calculated within the Distorted wave Born approximation (DWBA), starting as an unperturbed state with sharp interfaces or with the actual perpendicular profile of refraction index. Various kinds of simple geometrical shapes are available with a full account of size and shape distributions in the Decoupling Approximation (DA), in the Local Monodisperse Approximation (LMA) and also in the Size-Spacing Correlation Approximation (SSCA). Both, disordered systems of particles defined by their particle-particle pair correlation function and bi-dimensional crystal or para-crystal are considered.

Protein structural analysis
High resolution
  • Cryo-electron microscopy
  • X-ray crystallography
  • NMR
  • Electron crystallography
  • EPR
Medium resolution
  • Fiber diffraction
  • Mass spectrometry
  • SAXS
Spectroscopic
  • NMR
  • Circular dichroism
  • Dual Polarisation Interferometry
  • Absorbance
  • Fluorescence
  • Fluorescence anisotropy
Translational Diffusion
  • Analytical ultracentrifugation
  • Size exclusion chromatography
  • Light scattering
  • NMR
Rotational Diffusion
  • Fluorescence anisotropy
  • Flow birefringence
  • Dielectric relaxation
  • NMR
Chemical
  • Hydrogen-deuterium exchange
  • Site-directed mutagenesis
  • Chemical modification
Thermodynamic
  • Equilibrium unfolding
Computational
  • Protein structure prediction
  • Molecular docking
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