Yousaf Khan or Yousuf Khan may refer to:
- Yusaf Khan (born 1948), former Pakistan Army general and vice chief of army staff
- Yusuf Khan and Sherbano, Pashtun folklore akin to Romeo and Juliet
- Yousuf Khan (actor) (died 2009), Pakistani film actor
- Yousuf Khan (soccer player) (1937–2006), former Indian soccer player
- Dilip Kumar (born 1922), Indian actor, real name is Yusuf Khan
- Yusuf Khan (actor), Indian actor (different from Dilip Kumar)
- Yusef Khan, fictional character from EastEnders
- Yusef Khan, Iran, village in Iran
- Yousaf Ali Khan, British film director
- Yousuf Hussain Khan (1902–1976), historian, scholar and author
- Muhammed Yusuf Khan (1725–1764), Tamil soldier and later commandant of British East India Company troops