Nookie may refer to:
- Nookie, a drum and bass musician
- a slang term for sexual intercourse derived from the Cockney Rhyming Slang expression 'nook and cranny', meaning 'fanny', or British army slang from Arabic niki, "I fuck" - a slang term referring specifically to the vagina. (Note that in US slang 'fanny' instead refers to the buttocks, and 'Nookie' is mainly a slang term for 'vagina').
- "Nookie" (song), a 1999 song by Limp Bizkit
- Nookie Bear, a puppet handled by British ventriloquist Roger De Courcey
- Nookie, vocalist of Russian alternative nu metal band Slot_(band)
- Nittany Bank Nookie Monster, mascot of the State College Spikes