Do may refer to:
- The English verb do, which may serve as an auxiliary verb; see do-support
- Do (province) or circuit (道), the Korean and Japanese administrative division equivalent to the ancient Chinese dao
- Dō (philosophy) (道, "The Way"), the Japanese equivalent of the Chinese concept of the Tao
- 'do, a slang term for hairstyle (via "hairdo")
- Do (kana), a mora symbol in Japanese writing
- Do, prefix for Dornier Flugzeugwerke aircraft
- Dō (胴), the torso protection in a suit of kendo armor
- .do, the Dominican Republic's internet domain
- Do. or ditto marks
- Doctor of Osteopathic Medicine
- GNOME Do, an application launcher for Linux
In music:
- Do (musical note), the first note of the musical scale in solfege
- *Do (album), an album by Do
- Do, a type of buk (drum) used in Korean ritual music
- "Do", a song by The White Stripes from the 1999 album The White Stripes (album)
- The Dø, a French/Finish indie pop band
Surname:
- Do, Vietnamese surname Đỗ from the Chinese Du (杜)
- Do, Korean surname from the Chinese Du (surname) (都)
- Do, Korean surname from the Chinese Tao (surname) (陶)
- Do, Italian surname, from the Lombard name of the town Ono San Pietro
People:
- Khoa Do (杜), Vietnamese Australian actor
- D.O. (South Korean singer) (都), a member of the Chinese-South Korean boy band EXO
- Do (singer) or Dominique van Hulst, a Dutch singer
- Do, a cult name for Marshall Applewhite, leader of Heaven's Gate