UNIFI may refer to:
- UNIFI Companies, the company formed by the 2006 merger of Ameritas Acacia Mutual Holding Company and Union Central Life Insurance Company
- UNIFI (trade union), the British trade union that merged into Amicus in 2004
- The University of Florence
- The UNIversal FInancial messaging standard, more formally known as ISO 20022
- Unifi Network, a former division of PricewaterhouseCoopers made up of the Kwasha Lipton Group and its other benefits consulting divisions
- UniFi, a high speed broadband service in Malaysia