In enzymology, a trans-2-enoyl-CoA reductase (NAD+) (EC 1.3.1.44) is an enzyme that catalyzes the chemical reaction
- acyl-CoA + NAD+ trans-didehydroacyl-CoA + NADH + H+
Thus, the two substrates of this enzyme are acyl-CoA and NAD+, whereas its 3 products are trans-didehydroacyl-CoA, NADH, and H+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is acyl-CoA:NAD+ trans-2-oxidoreductase. This enzyme is also called trans-2-enoyl-CoA reductase (NAD+). This enzyme participates in butanoate metabolism.