Isolation
Samples of TSST-1 can be purified from bacterial cultures to use in in vitro testing environments, however this is not ideal due to the large number of factors that contribute to pathenogenesis in an in vivo environment. Additionally, culturing bacteria in vitro provides an environment which is rich in nutrients, in contrast to the reality of an in vivo environment, in which nutrients tend to be more scarce. TSST-1 can be purified by preparative isoelectric focusing for use in vitro or for animal models using a mini-osmotic pump.
Read more about this topic: Toxic Shock Syndrome Toxin
Famous quotes containing the word isolation:
“But your isolation must not be mechanical, but spiritual, that is, must be elevation.”
—Ralph Waldo Emerson (1803–1882)
“The only happy talkers are dandies who extract pleasure from the very perishability of their material and who would not be able to tolerate the isolation of all other forms of composition; for most good talkers, when they have run down, are miserable; they know that they have betrayed themselves, that they have taken material which should have a life of its own, to dispense it in noises upon the air.”
—Cyril Connolly (1903–1974)
“One who shows signs of mental aberration is, inevitably, perhaps, but cruelly, shut off from familiar, thoughtless intercourse, partly excommunicated; his isolation is unwittingly proclaimed to him on every countenance by curiosity, indifference, aversion, or pity, and in so far as he is human enough to need free and equal communication and feel the lack of it, he suffers pain and loss of a kind and degree which others can only faintly imagine, and for the most part ignore.”
—Charles Horton Cooley (1864–1929)