TOPO cloning is a molecular biology technique in which DNA fragments amplified by Taq polymerase are cloned into specific vectors without the requirement for DNA ligases. The Taq polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosin (A) to the 3'-end of the PCR products. This characteristic is exploited in TOPO-cloning.
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“Language is as real, as tangible, in our lives as streets, pipelines, telephone switchboards, microwaves, radioactivity, cloning laboratories, nuclear power stations.”
—Adrienne Rich (b. 1929)