Beyond Plant Disease
Potyvirus RNA codes for at least 7 different proteins. One of them is a protease. The TEV protease is a highly site-specific protease that biochemists have used to their advantage to create a protein purification system by incorporating TEV protease's recognition site into protein purification tags.
A gene construct is created containing the protein of interest fused to a TEV protease recognition site, followed by an affinity tag, such as a Polyhistidine-tag. Following affinity chromatography, the purified protein is then treated with TEV protease. TEV protease cleaves at its recognition site, removing the affinity tag. This allows for affinity purification of proteins that are not well-behaved when fused to protein tags. PMID 12963335
Read more about this topic: Tobacco Etch Virus
Famous quotes containing the words plant and/or disease:
“I please
To plant some more dew-wet anemones
That they may weep.”
—Unknown. The Thousand and One Nights.
AWP. Anthology of World Poetry, An. Mark Van Doren, ed. (Rev. and enl. Ed., 1936)
“Perfect Scepticisme ... is a disease incurable, and a thing rather to be pitied or laughed at, then seriously opposed. For when a man is so fugitive and unsettled that he will not stand to the verdict of his own Faculties, one can no more fasten any thing upon him, than he can write in the water, or tye knots in the wind.”
—Henry More (1614–1687)