TEV Protease

The TEV protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) (ENLYFQ(G/S)) and cleavage occurs between the Gln and Gly/Ser residues. Some of the advantages of this enzyme are its high specificity and its high activity rate. One of the main uses of this protein is for removing affinity tags from purified proteins. This peptidase belongs to the C4 peptidase family.

The molecular weight of this enzyme varies between 25 and 27 kDa depending on the specific construct used.

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Other articles related to "tev protease, protease, tev":

Tandem Affinity Purification - Process
... After washing, TEV protease is introduced to elute the bound material at the TEV protease cleavage site ... This second affinity step is required to remove the TEV protease as well as traces of contaminants remaining after the first affinity step ...
TEV Protease - External Links
... National Cancer Institute TEV FAQ Invitrogen's TEV Protease Product Page. ...
Tobacco Etch Virus - Beyond Plant Disease
... One of them is a protease ... The TEV protease is a highly site-specific protease that biochemists have used to their advantage to create a protein purification system by incorporating TEV ... gene construct is created containing the protein of interest fused to a TEV protease recognition site, followed by an affinity tag, such as a Polyhistidine-tag ...