Testin - Binding Partners

Binding Partners

TES does not appear to be an enzyme; rather it is a protein that mediates/regulates cellular functions via Protein:protein interactions. Pull down experiments reveal that TES has putative interactions mediated by the indicated domain:

Partner Domain ref Method
mENA/VASP LIM3 Yeast two Hybrid, Pull-down assay, Structure, ITC
Arp7a ??? Yeast two Hybrid
Zyxin LIM1 Yeast two Hybrid, Pull-down assay
Actin PET? Pull-down assay
α-Actinin PET? Pull-down assay
Paxillin PET? Pull-down assay

Garvalov et al. showed that the interaction between TES & zyxin were direct, using recombinant proteins expressed in E. coli.

Some of the potential binding partners (Zyxin, mENA) can be found in focal adhesion complexes; the range of binding partners indicates a potential role for TES in-between 'privileged' Actin polymerisation and focal adhesion contacts to the extracellular matrix. This tallies with the observation that GFP-tagged TES can be seen at focal adhesions.

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