Super Optimal Broth - Sterilization

Sterilization

Finally, the SOB medium should be autoclaved at 121 °C to ensure sterility. SOC medium should not be autoclaved because the high temperature can cause the glucose to react with tryptic peptides (see Maillard reaction). SOC can instead be filter sterilized through a 0.22 µm filter. Alternatively, SOB and magnesium and glucose additive solutions can be autoclaved separately and mixed afterwards to final concentrations.