Skybus may refer to:
- Skybus Airlines, an low-cost airline that operated in the United States between 2007 and 2008
- Skybus Super Shuttle, an airport bus service in Melbourne, Australia
- Skybus (Aqua Avia), a proposed privately owned airline in New Zealand during the 1970s
- Skybus Metro, rapid transit system in India.
- Isles of Scilly Skybus, British airline
- Transit Expressway Revenue Line, commonly known as "Skybus", a proposed people mover system in Pittsburgh, Pennsylvania