Single-molecule Experiment - Theoretical Notes

Theoretical Notes

• Single-molecule versus ensemble When looking on individual molecules, one usually can construct propagators, and jumping time probability density functions, of the first order, the second order and so on, where from bulk experiments, one usually obtain the decay of a correlation function. From the information contained in these unique functions (obtained from individual molecules), one can extract a relatively clear picture on the way the system behaves; e.g. its kinetic scheme, or its potential of activity, or its reduced dimensions form. In particular, one can construct (many properties of) the reaction pathway of an enzyme when monitoring the activity of an individual enzyme The problem with the analysis of single molecule data is filtering the noise.