Basic Protocol
- Perform microarray experiments — DNA microarray with oligo and cDNA primers, SNP arrays, protein arrays, etc.
- Input Expression Analysis in Microsoft Excel — see below
- Run SAM as a Microsoft Excel Add-Ins
- Adjust the Delta tuning parameter to get a significant # of genes along with an acceptable false discovery rate (FDR)) and Assess Sample Size by calculating the mean difference in expression in the SAM Plot Controller
- List Differentially Expressed Genes (Positively and Negatively Expressed Genes)
Read more about this topic: Significance Analysis Of Microarrays
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