Sequencer may refer to:
- Technology
- Electro-mechanical sequencer (Drum sequencer (controller), Cam timer, Piwheel controller), a system for controlling the sequence of events automatically
- DNA sequencer, a machine used to automatically produce a sequence readout from a biological DNA sample
- Microsequencer, a part of the control unit of a CPU
- Music sequencer, software or hardware device for recording, playing, and editing digital music data
- Video sequencer, software or hardware that changes between video feeds - such as those used in Closed-circuit television (CCTV) or digital video editing
- Protein sequencer, a machine used to automatically produce a sequence readout from a biological protein sample
- Arts and Entertainment
- Sequencer (Larry Fast album), 1976
- Sequencer (Covenant album), 1996
- Sequencer (Scenario album), 1983