SAM Riboswitch (S Box Leader) - Structure Organization

Structure Organization

The structure of the SAM riboswitch has been determined with X-ray crystallography. The SAM riboswitch is organized about a four way junction, with two sets of coaxially stacked helices arranged side-by-side. These stacks are held together by a pseudoknot formed between the loop on the end of stem P2 and the J3/4 joining region. The formation of the pseudoknot is facilitated by a protein-independent kink turn that induces a 100° bend into P2. Ribosomal proteins, known to bind kink-turns in the ribosome, favor SAM aptamer folding by interacting with P2 kink-turn motif. Both the kink-turn and the pseudoknot are critical to the establishment of the global fold and productive binding. The binding pocket is split between conserved, tandem AU pairs in stem P1, the conserved G in the J1/2 joining region, and the conserved asymmetric bulge in stem P3. The adenosyl and methionine main-chain moieties of S-Adenosyl methionine (SAM) are recognized through hydrogen-bonding into the bulge in P3 and the conserved G in J1/2. The methyl group is recognized indirectly through the charged sulfur, which forms an electrostatic interaction with the negative surface potential created by the tandem AU pairs in the minor groove of P1. These pairs are highly conserved and alterations to the orientation of these pairs, as well the identity of the bases in the pairs (i.e., GC pairs instead of AU pairs) result in reduced affinity for SAM. Affinity for SAH, however, is unaffected by changes to the P1 sequence, further supporting the idea that the interaction between SAM and the P1 helix is electrostatic in nature.

Other structural classes of SAM-binding riboswitches have been found that are entirely different from that of the SAM-I riboswitch. These unrelated SAM-binding riboswitches are SAM-II riboswitches, SAM-III riboswitches and SAM-SAH riboswitches. The details of these riboswitches are covered in their articles.

There are also classes of SAM-binding riboswitches that are structurally related to SAM-I riboswitches. SAM-IV riboswitches, appears to share a similar ligand-binding site with that of SAM-I riboswitches, but in the context of a discreet distinct scaffold. In SAM-IV, the P4 stem is located downstream of the P1 stem and interacts, by a second pseudoknot, with P3, while the kink turn is absent. SAM-I/IV variant riboswitches combine features of SAM-I and SAM-IV riboswitches. For example, they have the P4 outside of the multistem junction, like SAM-IV riboswitches, but also often have a SAM-I-like P4 within the multistem junction. However, unlike SAM-I and SAM-IV riboswitches, the variant riboswitches entirely lack an internal loop in P2. As noted above, this internal loop in SAM-I riboswitches forms a kink turn motif that allows the RNA to form a pseudoknot. The variant riboswitches lack an internal loop that might allow such a turn, and also show no signs of forming the SAM-I-like pseudoknot.