Entertainment
- Route 66 (TV series), a 1960s television program
- "Route 66 Theme", the TV series theme song
- Route 66 (film), a 1998 film
- VEB Film Leipzig, aka Route 66: An American (Bad) Dream; an open source film
- "Route 66" (song), also known as "(Get Your Kicks on) Route 66"
- The 2001 working title of the Pixar movie Cars
- Route 66 (band), see Jack Daniel (DJ)
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