Rosslyn can refer to:
- Roslin, Midlothian, Scotland
- Rosslyn, Arlington, Virginia, United States
- Rosslyn (Washington Metro), the Washington Metro station serving Rosslyn
- Rosslyn, Ontario, Canada
- Rosslyn (Edmonton), a neighborhood in the city of Edmonton, Canada
- Rosslyn, Gauteng, South Africa
- Rosslyn Academy, a school in Nairobi, Kenya
- Rosslyn Range, American long jumper