Commercialization
The company Pyrosequencing AB in Uppsala, Sweden commercialized machinery and reagents for sequencing short stretches of DNA using the pyrosequencing technique. Pyrosequencing AB was renamed to Biotage in 2003 which was acquired by Qiagen in 2008. Pyrosequencing technology was further licensed to 454 Life Sciences. 454 developed an array-based pyrosequencing technology which has emerged as a platform for large-scale DNA sequencing. Most notable are the applications for genome sequencing and metagenomics. GS FLX, the latest pyrosequencing platform by 454 Life Sciences (now owned by Roche Diagnostics), can generate 400 Mb in a 10 hour run with a single machine. Each run would cost about 5,000-7,000 USD.
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