Polidori is an Italian surname. Notable people with the surname include:
- Ambra Polidori, Mexican artist
- Frances Polidori, daughter of Gaetano, mother of Maria, Dante, William and Christina Rossetti
- Gaetano Polidori, Italian writer
- Giancarlo Polidori, Italian cyclist
- Gino Polidori, American politician
- John William Polidori, Italian-English physician and writer, son of Gaetano
- Paolo Polidori, Italian cardinal
- Robert Polidori, Canadian photographer
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