Plant Transformation Vector - Steps in Plant Transformation

Steps in Plant Transformation

  1. Propagate binary vector in E. coli
  2. Isolate binary vector from E.coli and engineer (introduce a foreign gene)
  3. Re-introduce engineered binary vector into E. coli to amplify
  4. Isolate engineered binary vector and introduce into Agrobacteria containing a modified (relatively small) Ti plasmid
  5. Infect plant tissue with engineered Agrobacteria (T-DNA containing the foreign gene gets inserted into a plant cell genome)
  6. In each cell T-DNA gets integrated at a different site in the genome
  • Note: There are many variations to these steps. A custom DNA plasmid sequence can be created and replicated in more than one way.

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