Steps in Plant Transformation
- Propagate binary vector in E. coli
- Isolate binary vector from E.coli and engineer (introduce a foreign gene)
- Re-introduce engineered binary vector into E. coli to amplify
- Isolate engineered binary vector and introduce into Agrobacteria containing a modified (relatively small) Ti plasmid
- Infect plant tissue with engineered Agrobacteria (T-DNA containing the foreign gene gets inserted into a plant cell genome)
- In each cell T-DNA gets integrated at a different site in the genome
- Note: There are many variations to these steps. A custom DNA plasmid sequence can be created and replicated in more than one way.
Read more about this topic: Plant Transformation Vector
Famous quotes containing the words steps and/or plant:
“And, beholding in many souls the traits of the divine beauty, and separating in each soul that which is divine from the taint which it has contracted in the world, the lover ascends to the highest beauty, to the love and knowledge of the Divinity, by steps on this ladder of created souls.”
—Ralph Waldo Emerson (1803–1882)
“The average parent may, for example, plant an artist or fertilize a ballet dancer and end up with a certified public accountant. We cannot train children along chicken wire to make them grow in the right direction. Tying them to stakes is frowned upon, even in Massachusetts.”
—Ellen Goodman (b. 1941)