Steps in Plant Transformation
- Propagate binary vector in E. coli
- Isolate binary vector from E.coli and engineer (introduce a foreign gene)
- Re-introduce engineered binary vector into E. coli to amplify
- Isolate engineered binary vector and introduce into Agrobacteria containing a modified (relatively small) Ti plasmid
- Infect plant tissue with engineered Agrobacteria (T-DNA containing the foreign gene gets inserted into a plant cell genome)
- In each cell T-DNA gets integrated at a different site in the genome
- Note: There are many variations to these steps. A custom DNA plasmid sequence can be created and replicated in more than one way.
Read more about this topic: Plant Transformation Vector
Famous quotes containing the words steps in, steps and/or plant:
“Now Morn her rosy steps in th’ eastern clime
Advancing, sowed the earth with orient pearl.”
—John Milton (1608–1674)
“People seldom see the halting and painful steps by which the most insignificant success is achieved.”
—Anne Sullivan (1866–1936)
“I grow savager and savager every day, as if fed on raw meat, and my tameness is only the repose of untamableness. I dream of looking abroad summer and winter, with free gaze, from some mountain-side,... to be nature looking into nature with such easy sympathy as the blue-eyed grass in the meadow looks in the face of the sky. From some such recess I would put forth sublime thoughts daily, as the plant puts forth leaves.”
—Henry David Thoreau (1817–1862)