Steps in Plant Transformation
- Propagate binary vector in E. coli
- Isolate binary vector from E.coli and engineer (introduce a foreign gene)
- Re-introduce engineered binary vector into E. coli to amplify
- Isolate engineered binary vector and introduce into Agrobacteria containing a modified (relatively small) Ti plasmid
- Infect plant tissue with engineered Agrobacteria (T-DNA containing the foreign gene gets inserted into a plant cell genome)
- In each cell T-DNA gets integrated at a different site in the genome
- Note: There are many variations to these steps. A custom DNA plasmid sequence can be created and replicated in more than one way.
Read more about this topic: Plant Transformation Vector
Famous quotes containing the words steps in, steps and/or plant:
“Now Morn her rosy steps in th’ eastern clime
Advancing, sowed the earth with orient pearl.”
—John Milton (1608–1674)
“What had really caused the women’s movement was the additional years of human life. At the turn of the century women’s life expectancy was forty-six; now it was nearly eighty. Our groping sense that we couldn’t live all those years in terms of motherhood alone was “the problem that had no name.” Realizing that it was not some freakish personal fault but our common problem as women had enabled us to take the first steps to change our lives.”
—Betty Friedan (20th century)
“They do not live in the world,
Are not in time and space.
From birth to death hurled
No word do they have, not one
To plant a foot upon,
Were never in any place.”
—Edwin Muir (1887–1959)