Steps in Plant Transformation
- Propagate binary vector in E. coli
- Isolate binary vector from E.coli and engineer (introduce a foreign gene)
- Re-introduce engineered binary vector into E. coli to amplify
- Isolate engineered binary vector and introduce into Agrobacteria containing a modified (relatively small) Ti plasmid
- Infect plant tissue with engineered Agrobacteria (T-DNA containing the foreign gene gets inserted into a plant cell genome)
- In each cell T-DNA gets integrated at a different site in the genome
- Note: There are many variations to these steps. A custom DNA plasmid sequence can be created and replicated in more than one way.
Read more about this topic: Plant Transformation Vector
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