Plant Transformation Vector - Plasmids Replication

Plasmids Replication

Plasmids replicate to produce many plasmid molecules in each host bacterial cell. The number of copies of each plasmid in a bacterial cell is determined by the replication origin. This is the position within the plasmids molecule where DNA replication is initiated. Most binary vectors have a higher number of plasmids copies when they replicate in E. coli, the plasmid copy-number is usually less when the plasmid is resident within Agrobacterium tumefaciens. Plasmids can also be replicated in the polymerase chain reaction (PCR).