Variants of -value Analysis
Other kinetic-perturbation techniques for analyzing the folding transition state have been developed in recent years. The most well-known variant is the -value, in which two metal-binding residues such as histidine are engineered into a protein; the folding kinetics are then studied as a function of the metal ion concentration. However, Fersht has illustrated some difficulties with this approach. An alternative "cross-linking" variant of the -value was developed in the course of studying the association of segments in the folding transition state through the introduction of covalent crosslinks such as disulfide bonds.
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