Palo santo (Spanish: "holy tree") can refer to:
- Lignum vitae, heartwood of tree species of the genus Guaiacum, native to subtropical and tropical regions of the Americas
- Bulnesia sarmientoi, a tree species native to the Gran Chaco area in South America
- Bursera graveolens, a tree species native to South America, used for incense, aromatic oil, and indigenous medicine
- Palo Santo, an album by indie rock band Shearwater
- Palo Santo Marron, a beer made by Dogfish Head Brewery