Nucleic Acid Structure Determination - RNA Sequencing

RNA Sequencing

While methods for each type of probing differ in steps, the probing of secondary structure involves certain steps in order to determine the structure. The first step involved is submitting the structural RNA to the probe of interest and incubating over a certain amount of time to allow the reaction to occur. The RNA is then transcribed using reverse transcriptase PCR, where this results in different lengths of bands due to the modification of the RNA at specific sites, which causes the reverse transcriptase to fall off. These bands are then run on a gel with sequencing data determined from RNA sequencing.