Nucleic Acid Methods

Nucleic acid methods are the techniques used to study nucleic acids (DNA and RNA).

Purification
  • Phenol-chloroform extraction
  • minicolumn purification
  • RNA extraction
  • Boom method
Quantification
  • Abundance in weight: spectroscopic quantification
  • Absolute abundance in number: Q-PCR
  • high-throughput relative abundance DNA microarray
  • high-throughput absolute abundance SAGE
  • Size: Gel electrophoresis
Synthesis
  • De novo: Oligonucleotide synthesis
  • Amplification: PCR
Kinetics
  • Dual polarization interferometry
  • QCM-D
Other
  • Nucleic acid simulations
  • DNA sequencing
  • Bisulfite sequencing
  • Expression cloning
  • Southern blot
  • Northern blot
  • Sucrose gradient centrifugation
  • Radioactivity in biological research
  • Lab-on-a-chip
  • Nuclear run-on assay
  • Fluorescent in situ hybridization
  • several Bioinformatics methods, such as RNA structure prediction

Read more about Nucleic Acid Methods:  See Also

Famous quotes containing the word methods:

    We are lonesome animals. We spend all our life trying to be less lonesome. One of our ancient methods is to tell a story begging the listener to say—and to feel—”Yes, that’s the way it is, or at least that’s the way I feel it. You’re not as alone as you thought.”
    John Steinbeck (1902–1968)