Measurement of NPQ
Non-photochemical quenching is measured by the quenching of chlorophyll fluorescence and is distinguished from photochemical quenching by applying a bright light pulse to transiently saturate photochemical quenching thus removing its contribution from the observed quenching. Non-photochemical quenching is not affected if the pulse of light is short. During the pulse, the fluorescence reaches the level reached in the absence of any photochemical quenching, known as maximum fluorescence, .
For further discussion, see Measuring chlorophyll fluorescence and Plant stress measurement.
Chlorophyll fluorescence can easily be measured with a chlorophyll fluorometer. Some fluorometers (such as the OS-5p) can calculate NPQ and photochemical quenching coefficients (including qP, qN, qE and NPQ), as well as light and dark adaptation parameters (including Fo, Fm, and Fv/Fm).
Read more about this topic: Non-photochemical Quenching
Famous quotes containing the words measurement of and/or measurement:
“That’s the great danger of sectarian opinions, they always accept the formulas of past events as useful for the measurement of future events and they never are, if you have high standards of accuracy.”
—John Dos Passos (1896–1970)
“That’s the great danger of sectarian opinions, they always accept the formulas of past events as useful for the measurement of future events and they never are, if you have high standards of accuracy.”
—John Dos Passos (1896–1970)