Nile Blue - Nile Blue in DNA Electrophoresis

Nile Blue in DNA Electrophoresis

Nile blue is also apparently used in a variety of commercial DNA staining formulations used for DNA electrophoresis. As it does not require UV trans-illumination in order to be visualised in an agarose gel as with ethidium bromide, it can be used to observe DNA as it is separated and also as a dye to aid in gel-extraction of DNA fragments without incurring damage by UV-irradiation.

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