Multiplex (assay) - "Multiplex" Versus "High-throughput"

"Multiplex" Versus "High-throughput"

Multiplex assays are often used in high-throughput screening settings, where many specimens can be analyzed using a multiplex (or other) assay. Strictly speaking, a multiplex assay is not necessarily high-throughput. When the execution of a single multiplex assay generates data for a large number of analytes (e.g., gene expression levels for all genes in the human genome), it is considered high-throughput. However, it is more the ability to rapidly process multiple specimens in an automated fashion that characterizes high-throughput techniques. Massive parallelization of assays is one way to achieve "high-throughput" status. Another way is via automating a manual laboratory procedure.

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