Steps in Molecular Cloning
In standard molecular cloning experiments, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into host organism, (6) Selection of organisms containing recombinant DNA, (7) Screening for clones with desired DNA inserts and biological properties.
Read more about this topic: Molecular Cloning
Famous quotes containing the words steps and/or cloning:
“I lie and wait for morning, and the birds,
The first steps going down the unswept street,
Voices of girls with scarves around their heads.”
—Philip Larkin (1922–1986)
“Language is as real, as tangible, in our lives as streets, pipelines, telephone switchboards, microwaves, radioactivity, cloning laboratories, nuclear power stations.”
—Adrienne Rich (b. 1929)