Background Correction
Depending on the type of array, signal related to nonspecific binding of the fluorophore can be subtracted to achieve better results. One approach involves subtracting the average signal intensity of the area between spots. A variety of tools for background correction and further analysis are available from TIGR, Agilent (GeneSpring), and Ocimum Bio Solutions (Genowiz).
Read more about this topic: Microarray Analysis Techniques
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